To confirm that Ptch1+/-Trp53-/- medulloblastoma have increased glycolysis, three P7 cerebella, three adult Ptch1+/+Trp53-/- cerebella and three adult Ptch1+/-Trp53-/- cerebella with tumors were analyzed for levels of hexokinase 2, the first rate-limiting enzyme in glycolysis by both immunohistochemistry and Western blot analysis. Here, PTCH1 is linked to medulloblastoma.