We found that TMPRSS4 expression was markedly enhanced in HCC tumors compared to corresponding adjacent nontumor tissues (p < 0.001, Fig. 4D), and positive staining of TMPRSS4 (score, 4–12) was detected in approximately 32.2% (128 of 398) of HCC samples. Here, TMPRSS4 is linked to hepatocellular carcinoma.