Closing of the wound was much faster in Lv-TMPRSS4 group than in control and Lv-GFP group (Fig. 2A), and the number of invading or attached cells of Lv-TMPRSS4 group was also increased several-fold over control and Lv-GFP group (Fig. 2B–D), indicating that the invasive activity of HCC cells was correlated with TMPRSS4 expression level. The gene discussed is TMPRSS4; the disease is hepatocellular carcinoma.