TGFB1 and embryonal rhabdomyosarcoma: Interestingly, our work suggests novel negative feedback mechanisms between hedgehog signaling and key muscle differentiation pathways, Wnt, Notch and TGFβ.26, 27, 28 Previous reports show that inhibition of Notch and TGFβ and activation of Wnt could lead to ERMS differentiation.7, 29, 30 Interestingly, activation of the Wnt pathway induced differentiation and reduced tumor initiation in a RAS-driven zebrafish ERMS model.