GLI1 and brain cancer: We also noted that alteration in NANOG expression led to a concordant change in the expression of GLI1 (Supplementary Figures 11a and c), which could be either due to the effect on overall proportion of ERMS stem cell-like population marked by GLI1 expression or the direct modulation of GLI1 expression as previously reported in brain cancers.21 Interestingly the expression of PDGFRA was also decreased upon NANOG knockdown (Supplementary Figure 11a).