To evaluate the role of NANOG on self-renewal and tumor growth independently, we generated stable rescue lines where NANOG expression was decreased in GLI1 overexpressing cells (GLI1+shNANOG) and corresponding empty vector (pCMV+pLKO.1) or GLI1 overexpression only controls (GLI1+pLKO.1) (Supplementary Figures 11d and e). This evidence concerns the gene GLI1 and neoplasm.