Taken together, these in vitro results indicated that NCTD alone or in combination with mF4-31C1 inhibited the lymphatic tube formation of HDLECs and the tumor lymphangiogenesis of HCACs by affecting these malignant phenotypes, inhibiting the expression of proliferating marker Ki-67 and anti-apoptotic gene Bcl-2 and induced S-phase cell cycle arrest, thus confirmed the anti-lymphangiogenic activity of NCTD. This evidence concerns the gene MKI67 and neoplasm.