CD40LG and toxoplasmosis: Our study showed that in the presence of IgM, an IgG titer cut-off value of ≥0.2 IU/mL met the definition of positivity if the patient was known previously seronegative for toxoplasmosis with IgG titer equal to 0 IU/mL; this cut-off was highly suggestive of early or proven toxoplasmosis seroconversion and able to confirm seroconversion in infected mothers several weeks earlier.