In the end of tumor protective assay, the tumor free survival rate of respective group also showed similar results, which is about 40% (CT26/GM-CSF/IL-18), 25% (CT26/GM-CSF), 15% (CT26), and less than 5% (CT26/IL-18 and PBS control) (Fig 5B and S5 Table). This evidence concerns the gene CSF2 and neoplasm.