Using a multiparameter analysis based on a negative depletion enrichment methodology, baseline values of 0–3969 CTCs/ml are reported for locally advanced SCCHN [3].Using a negative selection method for effective leukocyte depletion and enhanced detection of viable EpCAM positive and negative CTCs, the number are in the same scale with 0–3440 CTCs/ml [24]. Here, EPCAM is linked to head and neck squamous cell carcinoma.