All these results suggested that BORIS could affect the EMT process in luminal-like non-invasive breast cancer cells and indicated that BORIS may regulate some of the EMT-related genes, such as CDH1 (ECADH), SNAIL, TWIST and VIMENTIN. To confirm the acquisition of EMT phenotype of MCF7 cells after BORIS knockdown, we analyzed the migration capacity of these cells. The gene discussed is TWIST1; the disease is breast cancer.