In addition, after further defined analysis in the CIN subgroups, the LGCIN group did not show statistically meaningful negative correlation (Spearman's rho=−0.020, P=0.861), HGCIN (Spearman's rho=−0.133, P=0.045) and Cancer (Spearman's rho=−0.175, P=0.002) group showed a strong negative correlation between MKRN1 and PTEN. This evidence concerns the gene MKRN1 and cervical squamous intraepithelial neoplasia.