The combination of the eight biomarkers (serum iron + ferritin + Transferrin + TSAT+ serum soluble transferrin receptor + STfR/ferritin ratio + hepcidin + C-reactive protein) added no discriminatory diagnostic power for iron overload on MRI (AUC: 0.766; 95%CI: 0.697–0.835) by comparison with ferritin alone (AUC: 0.767; 95% CI: 0.698–0.835) (Fig 6). Here, HAMP is linked to Tangier disease.