The study screened 1,200 blood samples for hepatitis B virus (HBV) and aflatoxin (AF) biomarkers and recruited 124 residents who were both HBsAg and AF-albumin adducts positive, aged 20–55 with normal liver function test, serum alpha-fetoprotein negative, no personal history of cancer, and no use of prescribed medications. This evidence concerns the gene AFP and cancer.