In the present study we showed that co-culture of endothelial cells with DHA-treated breast cancer cells or application of microRNA mimics significantly inhibited tube formation and repressed the expression of pro-angiogenesis target genes, including PLAU and AMOTL1 for miR-23b and NRP1 and ETS2 for miR-320b. Here, NRP1 is linked to breast carcinoma.