To determine whether the five DLBCL-associated D2HGDH variants that we discovered affected the conversion of D2-HG into α-KG, we generated HEK-293 cells stably expressing the WT and mutant enzymes and used liquid chromatography-mass spectrometry (LC-MS) to quantify these metabolites. The gene discussed is D2HGDH; the disease is diffuse large B-cell lymphoma.