D2HGDH and B-cell non-Hodgkin lymphoma: As the D2HGDH variants identified are heterozygous, and a dominant negative effect is not obvious (compare the nearly indistinguishable measurements of empty vector versus mutant-expressing isogenic cells in multiple assays, (Fig. 2 and Supplementary Figs 4 and 7)), we used an siRNA strategy to partially knock down WT D2HGDH in two B-cell lymphoma cell lines and hence determine whether a hemizygous loss of D2HGDH would be of consequence.