NOS1 and ischemic stroke: ZL006 inhibited NMDA receptor-mediated NO synthesis, and showed robust neuroprotective properties in neurons and animal ischemic stroke models,24 but not when the nNOS gene was knocked out.24 ZL006 was described as being specifically de novo designed to create a compound that interacts with and disrupts the Asp62-Arg121 salt bridge via its carboxylic acid group.