When all groups were pooled for analysis, there was a significant positive correlation between the 7dpv-plasmablast frequency by FACS and 7dpv-influenza-specific plasmablasts detected by ELISPOT to be secreting IgG (r = 0.45, p = 0.02) or IgA (r = 0.57, p = 0.003) but not IgM (r = 0.24, p = 0.26) (Fig 2D–2F). This evidence concerns the gene CD40LG and influenza.