Our discovery of characterization of berberine as an antagonist of the TRPV4 channel would help in the elucidation of TRPV4 function and considered it as a clinical drug to target TRPV4 in treating the high-tension of vascular tone-related cardiovascular diseases, such as hypertension, vascular stiffness and stroke. The gene discussed is TRPV4; the disease is Hypertension.