The 5 proteins aside from type XIV collagen that were expressed in massive lymph node metastasis of breast cancer as determined by MRM analysis without an internal standard included 2 types of type I collagen, MSTP161, angiomotin and hexokinase type I. Like type XIV collagen, type I collagen and MSTP161 are extracellular matrix proteins. This evidence concerns the gene AMOT and metastatic malignant neoplasm in the lymph nodes.