Next, we found the OM or OM2 up-regulated ATGL protein expression was significantly abolished by pretreatment of the adipocytes with PGC1α siRNA (Fig 5D), Meanwhile, PGC1α siRNA treatment also blocked the elevated Pparα and Cpt1α mRNAs and mtDNA copy number induced by OM or OM2 (Fig 5E and 5F), indicating that PGC-1α played an importance role in fatty acid oxidation and mitochondrial biogenesis. The gene discussed is PNPLA2; the disease is ocular melanoma.