MIF and Cognitive impairment: However, we were interested to find that MIF cannot duplicate the effect of minocycline on MBP expression (Fig. 3C,D) and even aggravated cognitive impairment in object recognition test (Fig. 3E) and Morris water maze test (Fig. 3F, two-way ANOVA indicated a significant difference, P < 0.001) at 32 d after hypoperfusion.