PBMCs from at least 8 healthy donors and 3 pediatric sarcoma patients were nucleofected with SB100X transposase plus SB-IGF1R CAR (pKT2-CaIG and pKT2-CaIG:Z), SB-ROR1 CAR (pKT2-CaRG and pKT2-CaRG:Z), or without DNA as mock. Here, ROR1 is linked to sarcoma.