TARDBP and amyotrophic lateral sclerosis: When cells were incubated for 21 days, surprisingly, the formation of the intracellular TDP-43 aggregates and the mislocalization of TDP-43 from the nucleus to the cytoplasm were observed in the U251 cells of ALS-FTD-CSF group, but not in the ALS-CSF and NC-CSF groups, as shown in Figure 1A and 1B.