In the present study, we conducted an overall investigation of the potential factors that influence the prognosis of patients with colon cancer with a particular focus on genetic (somatic mutations and CIN/18q LOH) and epigenetic (LINE-1 hypomethylation, hMLH1 and hMSH2 promoter hypermethylation, and MSI status) changes and correlated these changes with certain established clinicopathological features. The gene discussed is MLH1; the disease is malignant colon neoplasm.