Herein, the involvement of HOTAIR promotion of liver cancer stem cell growth is supported by results from two parallel sets of experiments: (1) we clearly reveal that HOTAIR is overexpressed in human liver cancer tissue; (2) HOTAIR promotes human liver cancer stem cell malignant growth in vitro and in vivo. Evidently, our observations demonstrated that HOTAIR is crucial for cell growth and viability in liver cancer stem cells. The gene discussed is HOTAIR; the disease is liver cancer.