The difference in DNA methylation between RFS and n/RFS primary tumours was less considerable, although a statistically significant difference was observed for DAXX, P<0.0001; RXRA, P<0.01; C8orf46, P=0.01; NCOR2 and MSI2 (P<0.05; t-test) enhancer regions (Fig. 4). This evidence concerns the gene MSI2 and neoplasm.