MAP1LC3A and Alzheimer disease: Since these results supported usefulness of two-photon microscopic observation of EGFP-LC3 for evaluation of macroautophagy, we applied the technique to answer the questions whether fasting treatment induces macroautophagy in neurons and how the autophagic response is different between 5xFAD mice, one of the severest mouse AD models that firstly shows Aβ deposition at 3 months of age, and the background mice, C57BL/6 x SJL (Fig. 2).