DAX-1 was expressed in 31/38 ARMS (81.5%; 29 t+ and 2 t-), in 3/14 cases re-classified as ERMS, in 50% of mixed RMS (2/4) and of epithelioid RMS (1/2), and in the sclerosing RMS. This evidence concerns the gene NR0B1 and embryonal rhabdomyosarcoma.