Another approach to assess the role of TIMP-1 on insulin sensitivity was taken by Meissburger et al. [20], who found that injections with murine TIMP-1 in mice accelerated insulin resistance and increased hepatic triacylglycerol accumulation, suggesting that TIMP-1 has a causal role in development of decreased glucose tolerance and hepatic steatosis. This evidence concerns the gene INS and fatty liver disease.