Meanwhile, consistant with the results in the above, AKT1/2/3 and ERK1/2 phosphorylation was not inhibited with HHT treatment.To further examine the STAT3 phosphorylation in the xenograft tumor samples with different treatments, the tumor samples were frozen and cutted into 10 μm sections for fluorescent immunohistochemistry. This evidence concerns the gene AKT1 and neoplasm.