In HCC, Li’ et al showed that miR-451 upregulation led to downregulation of cyclin D1 and c-Myc through inhibition of NF-κB pathway initiated by direct targeting of the IKBKB 3′-untranslated region [21], but it is still unclear whether miR-451 can directly regulate the expression of c-Myc through binding to the promoter region of c-Myc. This evidence concerns the gene CCND1 and hepatocellular carcinoma.