Three short hairpin RNAs (pSil/shc-Myc #1, 2 or 3) were stably transfected into HCC cells, qRT-PCR and Western blotting confirmed that pSil/shc-Myc3 had the best silencing effect on c-Myc in HCC cells (Supplementary Figure 5a). Here, MYC is linked to hepatocellular carcinoma.