To further determine the roles of GSK-3β on the activity of snail promoter and the expression of snail protein, pcDNA/GSK-3β vector was previously constructed and the co-transfection of pcDNA/GSK-3β with pcDNA/miR-451 or pSil/shc-Myc#3 could partially restore the decreased activity of snail promoter and expression of total or nuclear snail protein in HCC cells induced by miR-451 upregulation or c-Myc downregulation (Figure 6e-6f). Here, SNAI1 is linked to hepatocellular carcinoma.