The luciferase reporter containing human snail promoter regions (2500 bp) from transcription start site were previously constructed to confirm the activity of snail promoter, and luciferase reporter analysis indicated that the transcriptional activity of snail promoter in both miR-451-upregulating and c-Myc-downregulating HCC cells was significantly inhibited (Figure 6c-6d). This evidence concerns the gene MYC and hepatocellular carcinoma.