The CLOTBUST trial (Combined Lysis of Thrombus in Brain Ischemia Using Transcranial Ultrasound and Systemic tPA) was a phase II, multicenter international randomized clinical trial that compared iv tPA vs. iv tPA plus 2-MHz transcranial Doppler monitoring for 2 h in acute ischaemic stroke patients with middle cerebral artery occlusion, within a 3 h time window. The gene discussed is PLAT; the disease is thrombotic disease.