No novel variants that co-segregated with disease in the family trio were detected at any of the 19 other known loci for Mendelian forms of POAG (GLC1A-G, GLC1I-K, GLC1M-Q, GLC3A-D) including the eight known causative genes namely, MYOC (GLC1A), CYP1B1 (GLC3A), WDR36 (GLC1G), ASB10 (GLC1F), OPTN (GLC1E), NTF4 (GLC1O), TBK1 (GLC1P), and LTBP2 (GLC3C). This evidence concerns the gene NTF4 and open-angle glaucoma.