In this study, we detected Flot-2 and EGFR proteins by immunohistochemistry (IHC) in 352 cases of NSCLC and 59 cases of non-cancerous lung control tissues, and investigated the correlations between the expression of Flot-2 and EGFR proteins and clinicopathological features and prognostic implications in NSCLC. Here, FLOT2 is linked to non-small cell lung carcinoma.