In Liu’s study in 2010, 922 PBC patients with AMA reactivity by IIF were tested using IgG/IgA dual isotype ELISA for detecting multiple mitochondrial antibodies (against recombinant antigens of the three enzymes of 2-OADC – MIT3) and nuclear autoantibodies specific for PBC (anti-sp100 and anti-gp210) (14). This evidence concerns the gene SP100 and primary biliary cholangitis.