The cell death induced by Rv1169c might not be apoptosis but necrosis since there is no difference in mRNA level of well-known apoptotic cell death related proteins such as bcl2 and caspase-1 (Figure S1) and cytokines TNF-α (Figure S2) and IL1β (Figure 8B) after both Ms_Rv1169c and Ms_Vec infection. The gene discussed is CASP1; the disease is infection.