At 24 h post-transfection into subconfluent, not-yet polarized Caco-2 colon carcinoma cells, HA-SLC26A3 WT is localized largely at the cell periphery and less so within the cells, whereas HA-SLC26A3 G393W is largely retained inside the cell. The gene discussed is SLC26A3; the disease is colon carcinoma.