To better describe the tumor suppressive signaling of miR-29b mimic in PTEN-mutant U251 cells, we examined the expression levels of proteins known to regulate this transition-including phosphorylated AKT (p-AKT), AKT, and ß-catenin in cells transfected with miR-29b mimic or miR-29b inhibitor by western blotting (Figure 2D). This evidence concerns the gene AKT1 and neoplasm.