Taking all the data together, we next sought to determine potential effects of pro-tumor serum mediators which could play an important role for thyroid tumor development; we used the Quantikine Mouse VEGF immunoassay and the IRMA assay in order to detect the serum level of vascular endothelial growth factor (VEGF) and Estradiol, respectively. The gene discussed is VEGFA; the disease is neoplasm.