Considering osteosarcoma is especially prevalent in children and young adults during quick osteoblastic differentiation [1, 2], unstable RB gene and p53 gene are commonly involved in this malignant transformation process [27]; we hypothesize that nsPEF affects osteosarcoma growth by targeting the Wnt/β-catenin signaling pathway, a key signaling cascade involved in osteosarcoma pathogenesis. This evidence concerns the gene RB1 and osteosarcoma.