In our analyses, a weak inverse trend was observed between sKlotho tertiles and CAC scores, which accords with an animal study describing higher vascular calcium content in Klotho-haploinsufficient mice with CKD versus wild type equivalents.[8] In contrast, sKlotho levels were not independently associated with the presence of cardiovascular disease in a Dutch sample of 127 dialysis patients.[39] Nevertheless, the current association between thyroid hormones and the calcification process implicates a role for fT3 in regulation of sKlotho. This evidence concerns the gene KL and cardiovascular disorder.