As a control, MCF-7 luminal breast cancer cells showed negative staining of basal markers (P63, CK14, Vim, CK5, CK17, N-cad) but positive staining of luminal markers CK18, CK8, E-cad, CK7, Muc1, ER, and PR (S1A Fig). Here, ESR1 is linked to breast carcinoma.