We addressed this question by placing 8 week-old congenic WT (C57B/6) and Plin2-null male mice, which had been maintained since weaning on a mouse chow diet (16% kcal fat, 24% kcal protein, 60% kcal carbohydrate), in metabolic chambers and feeding them ad libitum with calorically equivalent LF (10% kcal fat, 20% kcal protein, 70% kcal carbohydrate) or HF (60% kcal fat, 20% kcal protein and 20% kcal carbohydrate) diets (Table 1) for four days. This evidence concerns the gene PLIN2 and hydrops fetalis.