HK2 and neoplasm: We also measured the expression of hk2, ldha, pkm1 and pkm2 in both pre-tumors (one week after Dox induction) and HCC (three week after induction) from adult Myc/xmrk transgenic zebrafish and we found that hk2 and pkm2 showed up-regulation even from the pre-tumor stage while ldha and pkm1 were up-regulated only in the HCC stage (S1 Fig).These molecular data indicate an enhanced Warburg effect in the HCC stage.