Expression of fusion transcripts was confirmed by reverse transcription-PCR (RT–PCR) for 2 in-frame and 10 out-of-frame fusions (Supplementary Data 7), but no recurrent fusions were identified, except for the PAX3–FOXO1 fusion found in two cases of ARMS. The gene discussed is FOXO1; the disease is alveolar rhabdomyosarcoma.