EGF and anaplastic astrocytoma: Using cell culture conditions originally developed to promote in vitro growth of neural precursor cells from the neurogenic subependymal zone (serum-free medium supplemented with epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF)), Ignatova et al. described cells with stem-like features isolated from cortical glial tumors (anaplastic astrocytoma, WHO grade III and GBM, and WHO grade IV) [7].