INS and Alzheimer disease: In 3×Tg-AD mice fed with the HFD for 9 months, we have recently shown that an acute insulin injection (3.8 U/kg body weight) corresponding to approximately 2000-fold the normal fasting insulin level in the mouse, restores cortical soluble Aβ40 and Aβ42 back to the level of mice fed with the control diet (CD).