More importantly, administrations of orexins in the posterior aspect of the PVT (pPVT) was shown to increase anxiety whereas injections of the OX2R antagonist TCSOX229 was reported to have an acute anxiolytic effect in rats after a previous exposure to footshock stress (Li et al., 2010a,b; Heydendael et al., 2011). This evidence concerns the gene HCRTR2 and Anxiety.