Compared with the expression of cont-miR, miR-130a was significantly downregulated in the AGS cell line following transfection with the miR-130a inhibitor (Fig. 4A), and we found that RUNX3 expression was significantly increased in miR-130a-low-expressing gastric cancer cell lines, although Smad4 expression was not increased (Fig. 4B). This evidence concerns the gene RUNX3 and gastric cancer.