Using IFI16-EGFP constructs transfection in human osteosarcoma U2OS cells, Li et al., [15] studies showed that the two NLS motifs of IFI16 (aa 96–100 and aa 128–131) are essential for the entry of newly synthesized IFI16 in the cytoplasm to the normal cell nucleus. Here, IFI16 is linked to osteosarcoma.