Active caspase-1 (p20) was detected in the nucleus of infected HMVEC-d cells at 2 and 8 h post-infection demonstrating that the inflammasome is activated upon sensing KSHV in the nucleus, and the majority of activated caspase-1 was subsequently detected in the cytoplasmic fractions at later times of infection probably to prevent caspase-1 mediated adverse activities in the nucleus. Here, CASP1 is linked to infection.