Fig 4 shows the correlation between fasting plasma levels of 10- and 12-(Z,E)–HODE/LA and RBP4 (Fig 4A, p < 0.001), as well as glycoalbumin (Fig 4B, p < 0.001), which are both prominent biomarkers for the early detection of diabetes [5,6]. As 10- and 12-(Z,E)-HODE/LA alone can predict glucose tolerance, although not early-stage insulin resistance, we developed an algorithm to detect pre-diabetes using the above-mentioned markers. This evidence concerns the gene RBP4 and diabetes mellitus.