AKT1 and hepatocellular carcinoma: Furthermore, we assessed the effects of enforced expression of Ad-ANGPTL2 and Ad-LacZ on Akt phosphorylation in 3T3L1 adipocytes, C2C12 murine myoblasts, and HepG2 hepatocellular carcinoma cells, which did not effect on Akt phosphorylation (S9 Fig).